General description
Hot start polymerase chain reaction (PCR) is effortless using the FastStart™ PCR Master, a ready-to-use, double-concentrated hot start master mix. This master mix contains FastStart Taq DNA Polymerase, Roche high quality PCR-Grade nucleotides and all other reagents required for PCR and two-step reverse transcriptase (RT)-PCR on thermal cycler instruments.
FastStart™ PCR Master accepts modified nucleotides such as Digoxigenin (DIG)-dUTP. Labeling with DIG-dUTP can be achieved by adding the modified nucleotide in a final concentration of 10 μM to the final volume.
Combine this master mix with Roche′s Transcriptor First Strand cDNA Synthesis Kit to achieve excellent results in two-step RT-PCR.
Application
FastStart™ PCR Master has been used for the analysis of Nosema sp. DNA by polymerase chain reaction (PCR) method
, for validating the genomic DNA by PCR
, for amplification of RNA by PCR method.
The FastStart™ PCR Master is a convenient alternative to the single reagents for nearly all hot start PCR applications. It can be applied for the amplification of genomic DNA
and cDNA targets up to 2 kb long, or longer fragments on plasmid DNA targets, with high specificity, sensitivity, and yield.
The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. It can be used for:
- Hot Start PCR up to 2 kb
- Hot Start RT-PCR up to 2 kb
- High-throughput PCR
- Direct colony PCR
Features and Benefits
The FastStart Master has modified recombinant FastStart Taq DNA Polymerase, inactive at temperatures below +75°C. The kit also contains an optimized PCR buffer and GC-RICH Solution for handling a wide range of templates.
- Higher specificity, sensitivity, and yield:
Hot start PCR makes PCR setup easier.
- Use robotic setup.
Use this enyzme mix stable for 24 hours at +15 to +25°C.
- Reduce Setup time.
Store master mixes at +2 to +8°C for immediate use for up to one month.
Contents
2x concentrated master mix containing FastStart Taq DNA Polymerase, Reaction Buffer, and Nucleotides (dATP, dCTP, dGTP, dTTP).
Packaging
1 kit containing: 2x concentrated master mix containing FastStart Taq DNA Polymerase, Reaction Buffer, and Nucleotides (dATP, dCTP, dGTP, dTTP).
Unit Definition
Volume Activity: >= 80U/ml
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent Nos. 5,677,152 and 5,773,258, and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
FastStart is a trademark of Roche
